![]() OVA 257-264 -specific T-cell response and cytokine productionĪ Specific CD8 + T-cell (OT-I) proliferation in response to immature BMDCs incubated with fdOVA, fdOVA/sc-αDEC or OVA 257-264 peptide chemically coupled to anti-DEC-205 antibody (NLDC:pOVA). Published under the terms of the CC BY 4.0 license. These findings make fd bacteriophage a valuable tool for immunization without administering exogenous adjuvants.ĭEC‐205 TLR9 antigen delivery dendritic cells filamentous bacteriophage. ![]() Thus, phage particles containing a single-strand DNA genome rich in CpG motifs delivered via DEC-205 are able to intercept and trigger the active TLR9 innate immune receptor into late endosome/lysosomes and to enhance the immunogenicity of the displayed antigenic determinants. We also found that fdsc-αDEC is delivered into LAMP-1-positive compartments and co-localizes with TLR9. In agreement with these findings, we demonstrate that induction of proinflammatory cytokines and type I interferon by fdsc-αDEC was MYD88 mediated and TLR9 dependent. A significant differential expression of genes involved in innate immunity, co-stimulation and cytokine production was observed. In order to investigate the mechanisms of this activity, RNA-Sequencing of fd-pulsed dendritic cells was performed. 8 Institute of Protein Biochemistry, National Council of Research, Naples, Italy bacteriophage fd particles delivering antigenic determinants via DEC-205 (fdsc-αDEC) represent a powerful delivery system that induces CD8(+) T-cell responses even when administered in the absence of adjuvants or maturation stimuli for dendritic cells.8 Institute of Protein Biochemistry, National Council of Research, Naples, Italy PMC article.Buzzati-Traverso, National Council of Research, Naples, Italy Department of Science and Technology, University Parthenope of Naples, Naples, Italy. 7 Institute of Genetics and Biophysics A.6 Institute of Protein Biochemistry, National Council of Research, Naples, Italy Institute of Food Science, National Council of Research, Avellino, Italy.5 Department of Pediatric, Gynecological, Microbiological and Biomedical Sciences, University of Messina, Messina, Italy.4 Institute of Protein Biochemistry, National Council of Research, Naples, Italy Telethon Institute of Genetics and Medicine, Pozzuoli (NA), Italy.3 Telethon Institute of Genetics and Medicine, Pozzuoli (NA), Italy.Buzzati-Traverso, National Council of Research, Naples, Italy. 2 Institute of Genetics and Biophysics A.1 Institute of Protein Biochemistry, National Council of Research, Naples, Italy.
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